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KMID : 0390220040150010103
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Journal of Clinical Otolaryngology, Head and Neck Surgery
2004 Volume.15 No. 1 p.103 ~ p.108
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Detection of Human Papillomavirus using Polymerase Chain Reaction(PCR) in Nasopharyngeal Carcinoma
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Goh Eui-Kyung
Lee Byung-Joo
Lee Hyun-Sun
Cho Kyu-Sup
Roh Hwan-Jung
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Abstract
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Background and Objectives：Nasopharyngeal carcinoma (NPC) is an unique epithelial malignancy which occurs at a high frequency in certain regions of Southeast Asia. Epstein-Barr virus (EBV) has been proposed to be closely associated with NPC. Human papillomavirus (HPV) is associated with a proportion of upper aerodigestive tract carcinomas. We hypothesized that HPV might also contribute to the pathogenesis of NPC. This study attempted to detect HPV DNA in nasopharyngeal carcinoma by polymerase chain reaction (PCR). Materials and Methods：We reviewed thirty patients with nasopharyngeal carcinoma from 1995 to 2000 at the Department of Otolaryngology, Pusan National University Hospital. Thirty paraffin-embedded tissues were selected to PCR using type-specific primer pairs. Control group was selected twenty normal adenoid tissue. Results：HPV DNA was detected in 3/30 (10%) of NPC specimens, and in none of control specimens. WHO class I nasopharyngeal carcinomas (three cases) were all HPV type 6 positive. Conclusion：These results suggest that HPV type 6 may also be involved in the carcinogenesis of EBV-negative squamous cell nasopharyngeal carcinoma.
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KEYWORD
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Nasopharyngeal carcinoma, PCR, HPV
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